Efektivitas Giemsa sebagai Pengganti Pewarna Papanicolaou pada Sitologi Urin
DOI:
https://doi.org/10.62710/aqrn9h21Keywords:
Urine, Papanicolaou stain, Giemsa stain, CytologyAbstract
Papanicolaou staining is a common or most frequently used staining method in cytology because it provides a highly detailed view of cell nuclei and cytoplasmic transparency. Giemsa staining is generally used on blood smears, but it can also provide a highly detailed view of cells, including epithelial cells. This study aims to investigate and evaluate the effectiveness of Giemsa staining as a substitute for Papanicolaou staining in urinary cytology. The research method employed was a descriptive experimental laboratory study evaluating twenty urine cytology slides stained with a 25% Giemsa solution, using Papanicolaou staining as the reference method. The slides were evaluated based on cell morphology, nuclear contrast, cytoplasmic contrast, and background clarity. Most of the urine cytology slides stained with Giemsa were classified as “Good” (50%) or “Very Good” (35%), with no slides classified as “Not Good.” Giemsa staining preserves nuclear morphology but shows lower cytoplasmic transparency compared to Papanicolaou staining.
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Alberto, P., & Margarita, A. (2014). Correlation between the cytology of urine sediment in fresh sample and smears stained by Papanicolaou and Giemsa methods. Journal of Cytology, 31(1), 25. https://doi.org/10.4103/0970-9371.130666
Ardina, R., & Rosalinda, S. (2018). Morfologi Eosinofil Pada Apusan Darah Tepi Menggunakan Pewarnaan Giemsa, Wright, dan Kombinasi Wright-Giemsa. Jurnal Surya Medika, 3(2), 5–12. https://doi.org/10.33084/jsm.v3i2.91
Bisharah, S., Raap, M., & Abbas, M. (2025). Urine Cytological Diagnostics: Possibilities and Limitations—A 25-Year Review and Overview at Hannover Medical School. Clinics and Practice, 15(12), 234. https://doi.org/10.3390/clinpract15120234
Brilhante-Simões, P., Lopes, R., Delgado, L., Machado, A., Silva, A., Martins, Â., Marcos, R., Queiroga, F., & Prada, J. (2025). What Comes from Cytology Diagnosis: A Comprehensive Epidemiological Retrospective Analysis of 3068 Feline Cases. Veterinary Sciences, 12(7), 671. https://doi.org/10.3390/vetsci12070671
Charisma, A. M., Permatasari, M. I., & Negara, Y. A. K. (2025). Effectiveness of Using Methylene Blue Dyes in Papanicolaou Staining in Cytology of Ascites Fluid. Jurnal Ilmu Dan Teknologi Kesehatan, 12(2), 175–181. https://doi.org/10.32668/jitek.v12i2.1803
Chen, F., & Lin, X. (2023). The Paris System for Reporting Urinary Cytology: An Updated Review. Journal of Clinical and Translational Pathology, 3(2), 59–74. https://doi.org/10.14218/jctp.2022.00035
Cortes, G. A., & Flores, J. L. (2020). Physiology, Urination. PubMed; StatPearls Publishing.
Damanik, E. M. Br., Manafe, D. R. T., & Setianingrum, E. L. S. (2020). Prevalensi Risiko Tinggi Displasia Cerviks Pada Metode Iva Positif Dan Papsmear Di Puskesmas Bakunase Kota Kupang. Cendana Medical Journal, 8(1), 394–402. https://doi.org/10.35508/cmj.v8i1.2642
Dila, T. R., Raharjo, E. N., & Rukmana, D. I. (2023). Perbandingan Pewarnaan Giemsa, Diff Quick Dan Papanicolaou Preparat Efusi Pleura Di Rsud A.W Sjahranie. Jurnal Kesehatan Tambusai, 4(3), 4252–4258. https://doi.org/10.31004/jkt.v4i3.18768
Feng, X., Wang, Y., Zhao, L., Zhang, K., Jing, W., Guo, L., Wang, J., Liu, Y., Hou, H., Shi, G., & Li, Q. (2024). Comparing Haematoxylin-eosin with Papanicolaou Stain for Demonstration of Endometrial Cytological Examination. Authorea (Authorea). https://doi.org/10.22541/au.170668600.06669457/v1
Franjic, S. (2023). Urinalysis Can Indicate the Presence of a Pathological Condition. Journal of Urology and Research, 10(1), 1–5. https://doi.org/10.47739/2379-951X/1141
Ilegbedion, I. G., Sylvanus, B., & Uchenna, E. E. (2021). Comparative Study of Gynaecological and Non-Gynaecological Samples Received in Igbinedi on University Teaching Hospital. The International Journal of Science & Technoledge, 9(8), 52–57. https://doi.org/10.24940/theijst/2021/v9/i8/st2108-017
Kakati, P. K., & Barman, R. (2026). A Comparative Study of Ultrafast Papanicolaou Staining with Standard Papanicolaou Staining Technique for the Assessment of Cervical Smear – A Hospital Based Observational Study. Zenodo, 7(2), 1766–1769. https://doi.org/10.5281/zenodo.19576902
Kars, M., Çetin, M., Toper, M. H., Filinte, D., & Çam, K. (2024). Contemporary Role of Urine Cytology in Bladder Cancer. The Bulletin of Urooncology, 23(3), 73–77. https://doi.org/10.4274/uob.galenos.2024.2024.3.3
Kral, M., Zemla, P., Hradil, D., Skotak, H., Hartmann, I., Langova, K., Bouchal, J., & Kurfurstova, D. (2022). Iodinated Contrast Medium Affects Urine Cytology Assessment: A Prospective, Single-Blind Study and Its Impact on Urological Practice. Diagnostics (Basel, Switzerland), 12(10), 2483. https://doi.org/10.3390/diagnostics12102483
Kumala, I., Triswanti, N., Hidayat, H., & Terta, R. L. (2022). Gambaran Hasil Pemeriksaan Urinalisis Pada Pasien Infeksi Saluran Kemih Yang Terpasang Kateter Di Ruang Rawat Inap Penyakit Dalam Rsud Dr. H. Abdul Moeloek Provinsi Lampung. Jurnal Medika Malahayati, 7(1), 276–285. https://doi.org/10.33024/jmm.v6i1.6187
lhotu-owoicho, A., & Singh, A. P. (2022). Importance Of Papanicolaou Staining In Gynecologic Cytology. EPRA International Journal of Multidisciplinary Research (IJMR), 8(9), 216–219. https://doi.org/10.36713/epra2013
Pamusok, J. A., Hutasoit, G. A., Syamsi, N., & Wahyuni, R. D. (2026). Perbandingan Kualitas Preparat Sitopatologi Cairan Efusi Pleura Dengan Pewarnaan Giemsa Dan Papanicolau. Medika Alkhairaat: Jurnal Penelitian Kedokteran Dan Kesehatan, 8(01), 1478–1484. https://doi.org/10.31970/ma.v8i01.424
Prihatin, I., Santosa, B., & Wibisono, F. (2020). Comparison of Pap Smear Preparation Quality Containing Orange G and Without Orange G on the Results of the Papanicoulou Painting. Biomedika, 13(1), 40–45. https://doi.org/10.31001/biomedika.v13i1.775
Raju, K. (2016). Evolution of Pap Stain. Biomedical Research and Therapy, 3(2), 490–500. https://doi.org/10.7603/s40730-016-0006-8
Sari, I., Darwin, B., & Realita, T. E. (2021). Analisa Metode Fiksasi Kering Menggunakan Giemsa Dan Fiksasi Basah Menggunakan Papanicolaou Pada Pemeriksaan Pap Smear. Masker Medika, 9(1), 446–454. https://doi.org/10.52523/maskermedika.v9i1.456
Sathawane, P., Kamal, M. M., Deotale, P. R., & Mankar, H. (2022). Nuances of the Papanicolaou Stain. CytoJournal, 19(43), 1–7. https://doi.org/10.25259/CMAS_03_18_2021
Trisna, M., Indanah, Rusidah, Y., Saputro, A. A., Sholikhati, A., & Ekawati, S. N. (2026). Parijoto (Medinilla speciosa) extract as a natural dye alternative for peripheral blood smears: A comparative study with giemsa stain. Journal of Indonesian Medical Laboratory and Science (JoIMedLabS), 7(1), 1–11. https://doi.org/10.53699/joimedlabs.v7i1.352
Vap, L. M., & Shropshire, S. B. (2017). Urine Cytology. Veterinary Clinics of North America: Small Animal Practice, 47(1), 135–149. https://doi.org/10.1016/j.cvsm.2016.07.009
Yıldırım, H., Korkmaz, M., Göker, A., Demirci, H., & Güvenal, T. (2019). A comparative study for selectivity of micronuclei in cervical exfoliated cells on chronic boron effects. Journal of Cytology, 36(2), 75–78. https://doi.org/10.4103/joc.joc_185_17
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Copyright (c) 2026 Jesslyn Widya Salim, Christian Dame Aditya, Christine Vania Purba, Kasamira Sukacita Uliarti Sau, Diana Intan Gabriella Lusiana, Fransisca Probo Setyoningrum (Author)
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