Komparasi Efektivitas Minyak Pinus dan Minyak Alpukat Sebagai Pengganti Xylene Dalam Proses Penjernihan (Clearing) Histologi
DOI:
https://doi.org/10.62710/78vbt377Keywords:
Minyak pinus, Minyak Alpukat, Xylene, Clearing Histologi, Kualitas preparat, Pine oil, Avocado oil, Xylene, Histological Clearing, Specimen qualityAbstract
Xylene is a standard clearing agent in histological tissue processing; however, its use poses a toxicity risk to the health of laboratory staff, making it necessary to find safer natural alternatives, such as pine oil and avocado oil. This experimental laboratory study aimed to compare the effectiveness of pine oil and avocado oil as xylene substitutes in improving the quality of mouse liver histological preparations. A total of twenty-seven mouse liver tissue sections were divided into three treatment groups: a xylene control group, an avocado oil group, and a pine oil group. The sections were then stained using the hematoxylin-eosin method and evaluated using a scoring system that assessed nuclear chromatin integrity, cytoplasmic contrast, preservation of tissue architecture, and microtome section quality. The results showed that pine oil achieved the highest total score, with mechanical performance comparable to xylene and superior preservation of cellular structure, while avocado oil demonstrated good cytoplasmic contrast and nuclear integrity but had limitations in section quality due to its high viscosity. Xylene itself excelled in ease of sectioning but resulted in poor nuclear integrity and cytoplasmic contrast due to repeated use. This study concludes that pine oil is the most effective and environmentally friendly alternative clarifying agent, while avocado oil still requires further process optimization before it can be widely recommended.
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Bright, O. A., Samuel, D. N., Musah Ayeley Adisa, Dorcas, O. O., & Ebenezer Senu. (2024). Clearing Properties Between Coconut Oil and Xylene in Histological Tissue Processing. Journal of Histochemistry and Cytochemistry, 72(4), 221554241241369. https://doi.org/10.1369/00221554241241369
D'Azzuri, B. M., Susianti, S., & Karima, N. (2023). Vegetable oils as a substitute of toxic chemicals in the histopathological clearing process. Medical Profession Journal of Lampung, 13(6), 1080–1083. https://doi.org/10.53089/medula.v13i6.813
Devendrababu, N., et al. (2026). Safer and sustainable alternatives to xylene in histopathology: A comprehensive review. Journal of Applied Toxicology. https://doi.org/10.1002/jat.70029
Doring Krumreich, F., Kuhn Rutz, J., Do Espirito Santo de Jesus, F., Lubke Buss, G., Barboza Mendonça, C. R., Dellinghausen Borges, C., & Zambiazi, R. C. (2026). Rheological evaluation of Avocado OILS subjected to different extraction processes. Desafios - Revista Interdisciplinar Da Universidade Federal Do Tocantins, 13(1), 43–54. https://doi.org/10.20873/vol13n120263
Gopinath, A., & Yadav, S. A. (2026). Comparative Analysis of Essential Oil Composition in Pinus sylvestris, Leucadendron argenteum, Impatiens balsamina and Abies sibirica. Current Analytical Chemistry, 22(3), 544-554.
Choukri, H., Harhar, H., Lee, L. H., Mani, V., Ming, L. C., Goh, K. W., Bouyahya, A., & Tabyaoui, M. (2023). Proximate composition, lipid and elemental profiling of eight varieties of avocado (Persea americana). Scientific Reports, 13(1), Article 22767. https://doi.org/10.1038/s41598-023-50119-y
Niaz, K., Bahadar, H., Maqbool, F., & Abdollahi, M. (2015). A review of environmental and occupational exposure to xylene and its health concerns. EXCLI journal, 14, 1167.
Ningombam Jyostna, Bhargava, D., & Bansal, P. (2024). Use of Carrot Oil, Olive Oil, Pine Oil, Rose Oil in Comparison to Xylene as Clearing Agent During Tissue Processing: an in Vitro Study. Biosciences Biotechnology Research Asia, 21(3), 1111–1120. https://doi.org/10.13005/bbra/3290
S Kim Suvarna, Layton, C., & Bancroft, J. D. (2013). Bancroft’s theory and practice of histological techniques (7th ed.). Churchill Livingstone Elsevier.
Singh, P., Dave, A., Arora, M., Madan, P. S., & Rai, R. (2023). Evaluation of the efficacy of hematoxylin and eosin stain when xylene is completely replaced by turpentine or kerosene oil—A comparative study for oral tissues. Indian Journal of Pathology & Microbiology, 66(4), 775–779. https://doi.org/10.4103/ijpm.ijpm_389_22
Snoddy, E. O., Nguyen, C., & Visbal, A. P. (2025). PINE OIL AND LEMON OIL AS VIABLE BIOSAFE XYLENE ALTERNATIVES FOR HISTOLOGICAL STAINING IN TEACHING AND RESEARCH LABORATORIES. The Texas Journal of Science, 77(1). https://doi.org/10.32011/txjsci_77_1_article5
Sofyanita, S., Nuraini, E., & Kusuma, A. (2022). Pengaruh penggunaan minyak nabati sebagai bahan clearing terhadap kualitas preparat histologi. Jurnal Labora Medika.
Tanwar, M., Sharma, M., Karthikeyan, R., Kumar, M., & Tyagi, V. (2022). Usage of coconut oil as a biofriendly xylene substitute in tissue processing and staining. International Journal of Health Sciences, 6(S1), 660–675. https://doi.org/10.53730/ijhs.v6nS1.4813
Thamilselvan, S., Sherlin, H. J., Jayaraj, G., Don, K. R., & Santhanam, A. (2021). Cedarwood oil as an alternative to xylene as a clearing agent in histopathological tissue processing—A comparative study. Journal of Oral and Maxillofacial Pathology, 25(2), 299–305. https://doi.org/10.4103/0973-029X.325232
Wimpy, Endang Widhiyastuti, Astri Aditya Wardhani, Pratama, E. O., Khoirunisa Saputri, & Ardy Prian Nirwana. (2025). Histological evaluation of eco-friendly clearing agents as substitutes for xylol in hematoxylin and eosin staining of fibroadenoma mammae tissue. Jurnal Teknologi Laboratorium, 14(2), 253–266. https://doi.org/10.29238/teknolabjournal.v14i2.698
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Copyright (c) 2026 Joy Miracle Tumigolung Kawer, Yessica Stefanie Cheriananda, Margareth Tumansery, Alessandro Stefano Kamengon, Diana Intan Gabriella Lusiana, Fransisca Probo Setyoningrum (Author)
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